Skip to Content
Merck
  • Redox signalling to nuclear regulatory proteins by reactive oxygen species contributes to oestrogen-induced growth of breast cancer cells.

Redox signalling to nuclear regulatory proteins by reactive oxygen species contributes to oestrogen-induced growth of breast cancer cells.

British journal of cancer (2015-05-13)
V O Okoh, N A Garba, R B Penney, J Das, A Deoraj, K P Singh, S Sarkar, Q Felty, C Yoo, R M Jackson, D Roy
ABSTRACT

17β-Oestradiol (E2)-induced reactive oxygen species (ROS) have been implicated in regulating the growth of breast cancer cells. However, the underlying mechanism of this is not clear. Here we show how ROS through a novel redox signalling pathway involving nuclear respiratory factor-1 (NRF-1) and p27 contribute to E2-induced growth of MCF-7 breast cancer cells. Chromatin immunoprecipitation, qPCR, mass spectrometry, redox western blot, colony formation, cell proliferation, ROS assay, and immunofluorescence microscopy were used to study the role of NRF-1. The major novel finding of this study is the demonstration of oxidative modification of phosphatases PTEN and CDC25A by E2-generated ROS along with the subsequent activation of AKT and ERK pathways that culminated in the activation of NRF-1 leading to the upregulation of cell cycle genes. 17β-Oestradiol-induced ROS by influencing nuclear proteins p27 and Jab1 also contributed to the growth of MCF-7 cells. Taken together, our results present evidence in the support of E2-induced ROS-mediated AKT signalling leading to the activation of NRF-1-regulated cell cycle genes as well as the impairment of p27 activity, which is presumably necessary for the growth of MCF-7 cells. These observations are important because they provide a new paradigm by which oestrogen may contribute to the growth of breast cancer.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MISSION® esiRNA, targeting mouse Nrf1
Pricing and availability is not currently available.
Sigma-Aldrich
Amyloid Protein Non-Aβ Component, ≥80% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
5-(Iodoacetamido)fluorescein, ≥90% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Akt1
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Mecp2
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting human COPS5
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
DL-Methionine, 99%, FCC, FG
Pricing and availability is not currently available.
Sigma-Aldrich
DL-Cysteine, technical grade
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Pricing and availability is not currently available.
Sigma-Aldrich
DL-Methionine, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
Pricing and availability is not currently available.
Sigma-Aldrich
Tamoxifen, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting human MECP2
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting human AKT1, RP11-982M15.2
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting human NRF1
Pricing and availability is not currently available.
Sigma-Aldrich
MISSION® esiRNA, targeting human PITX2
Pricing and availability is not currently available.
Sigma-Aldrich
DL-Methionine, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Pricing and availability is not currently available.