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  • Abiotrophia defectiva bleb-associated endophthalmitis confirmed with 16s ribosomal RNA sequencing.

Abiotrophia defectiva bleb-associated endophthalmitis confirmed with 16s ribosomal RNA sequencing.

Journal of glaucoma (2013-08-24)
Ming-Han Hugo Lee, Mitchell Lawlor, Anne J Lee
ABSTRACT

One recognized complication of trabeculectomy with visually devastating potential is blebitis. We present a case of a 74-year-old woman with a culture and polymerase chain reaction-positive Abiotrophia defectiva bleb-associated endophthalmitis. Abiotrophia defectiva is a rare but possible cause of endophthalmitis secondary to blebitis and should be considered in culture-negative cases. Prompt identification, hence directed eradication, of the causative organism in such visually threatening cases may be facilitated by requesting polymerase chain reaction and 16S ribosomal RNA sequencing.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cephalothin sodium salt
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Ceftazidime pentahydrate, United States Pharmacopeia (USP) Reference Standard
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Supelco
Atropine solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Atropine for peak identification, European Pharmacopoeia (EP) Reference Standard
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Vancomycin hydrochloride from Streptomyces orientalis, ≥85% (Vancomycin B)
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Ceftazidime for peak identification, European Pharmacopoeia (EP) Reference Standard
Cefalotin for impurity B identification, European Pharmacopoeia (EP) Reference Standard