Chymotrypsin: molecular and catalytic properties.

Chymotrypsin: molecular and catalytic properties.

Clinical biochemistry (1986-12-01)

W Appel

ABSTRACT

A review on the peptide hydrolase chymotrypsin gives some characteristics concerning the structure, specificity, mechanism of activation from its precursor and catalytic activity. Problems of the active sites, allosteric alterations, changes of conformation, different behaviour against types of substrates, and suggested kinetics are shown in some detail. Finally, the diagnostic role of this serine proteinase is mentioned.

MATERIALS

Product Number

Brand

Product Description

C2160000

Chymotrypsin, BRP, European Pharmacopoeia (EP) Reference Standard

C3142

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

C6423

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder

C4129

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas, Type II, lyophilized powder, ≥40 units/mg protein

C7762

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas, Type I-S, essentially salt-free, lyophilized powder

C8946

Sigma-Aldrich

α-Chymotrypsin from human pancreas, lyophilized powder

CHY5S

Sigma-Aldrich

α-Chymotrypsin from bovine pancreas, ≥40 units/mg protein, vial of 5 mg