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  • Metabolic engineering of Rhizopus oryzae for the production of platform chemicals.

Metabolic engineering of Rhizopus oryzae for the production of platform chemicals.

Applied microbiology and biotechnology (2012-04-25)
Bas J Meussen, Leo H de Graaff, Johan P M Sanders, Ruud A Weusthuis
ABSTRACT

Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over the pathways leading to the formation of these products. These platform chemicals are produced in high yields on a wide range of carbon sources. The yields are in excess of 85 % of the theoretical yield for L: -(+)-lactic acid and ethanol and over 65 % for fumaric acid. The study and optimization of the metabolic pathways involved in the production of these compounds requires well-developed metabolic engineering tools and knowledge of the genetic makeup of this organism. This review focuses on the current metabolic engineering techniques available for R. oryzae and their application on the metabolic pathways of the main fermentation products.

MATERIALS
Product Number
Brand
Product Description

Supelco
Fumaric acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Pricing and availability is not currently available.
Sigma-Aldrich
Fumaric acid, FCC, FG
Pricing and availability is not currently available.
Fumaric acid, European Pharmacopoeia (EP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Fumaric acid, ≥99.0% (T)
Pricing and availability is not currently available.