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  • Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.

Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.

Acta crystallographica. Section F, Structural biology and crystallization communications (2012-07-04)
Kayla Baretta, Craig Garen, Jiang Yin, Michael N G James
ABSTRACT

Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5 Å using synchrotron radiation at the Canadian Light Source.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae), ammonium sulfate suspension, ≥500 units/mg protein
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50 mL
1 Estimated to ship on February 23, 2025
€102.00

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