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  • Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.

Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.

Journal of clinical microbiology (1989-04-01)
G J Delisle, A Ley
ABSTRACT

A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to 90%, and the specificity was 100%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, tablet
Pricing and availability is not currently available.
Sigma-Aldrich
Indoxyl β-D-glucuronide cyclohexylammonium salt
Pricing and availability is not currently available.