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  • A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses.

A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses.

Cellular microbiology (2012-01-12)
José J Rodríguez-Herva, Pablo González-Melendi, Raquel Cuartas-Lanza, María Antúnez-Lamas, Isabel Río-Alvarez, Ziduo Li, Gema López-Torrejón, Isabel Díaz, Juan C Del Pozo, Suma Chakravarthy, Alan Collmer, Pablo Rodríguez-Palenzuela, Emilia López-Solanilla
ABSTRACT

The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His(6) -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1(D299A) non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant immunity responses and a target for pathogen suppression.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat, 1.0 mg/mL, clone MBP 7G4, purified immunoglobulin
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