Duolink^® Proximity Ligation Assay

Duolink^® Proximity Ligation Assays (PLA) enable the studying of endogenous Protein-Protein Interactions (PPI) and Post-Translational Modifications, providing a fluorescent signal for a single event that can be visualized by either microscopy or flow cytometry. To study Protein-Protein Interactions, Duolink^® PLA technology uses a pair of antibodies labeled with oligonucleotides which when present within 40 nm undertake rolling circle amplification to generate a specific fluorescent signal after the addition of labeled probes.

Available in four different colors, for both flow cytometry and microscopy, Duolink^® PLA allows for the visualization of multiple Protein-Protein Interactions per cell. Uncover the opportunities in your research with amplified performance and the benefits of Duolink^® PLA technology: 

• Visualize protein interactions – both stable and transient
• Visualize post-translational modifications
• Detect endogenous proteins – no overexpression or genetic manipulation
• High specificity – use of two antibodies/probes eliminates false positives
• No special equipment needed – standard immunofluorescence and flow cytometry-based methods available

Duolink^® PLA Products

• Duolink^® PLA Starter Kits contain all the necessary reagents you need to perform a fluorescent experiment and analyze up to 30 samples.
• Duolink^® Probemaker Kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody.
• Duolink^® PLA Multicolor Kits allow for multiple PPI visualizations by microscopy.
• Duolink^® flowPLA Detection Kits enable flow cytometry applications using four colors: green, orange, red, and far red.
• Duolink^® PLA Brightfield Detection contains oligonucleotides labeled with horseradish peroxidase (HRP) allowing use with a light microscope.