Bioprocessing Cell Lines
A cell line that maintains favorable growth and productivity characteristics is vital for biopharmaceutical manufacturing processes, whether you are producing monoclonal antibodies, recombinant proteins, or viral vectors for gene therapies. Our ready-to-use cell line systems can accelerate your upstream development by greatly reducing the time, cost, and risk of developing new cell lines. Our cell lines are rationally engineered and designed for compliant biomanufacturing.
Key features include:
- Fully traceable cell lines with comprehensive documentation
- cGMP banked and characterized
- Paired, optimized, and chemically defined media and feeds
- Comprehensive user protocols for efficient cell line development and seamless scale-up, supported by technical consultation
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Products
Related Product Resources
- Flyer: Sf-RVN® Platform
With the Sf-RVN® Platform, we provide a rhabdovirus-free cell line that improves the safety profile of your bioprocesses combined with a chemically defined medium specifically formulated to get excellent growth and productivity
- Product Information Sheet: Sf-RVN® Insect Cell Line
Sf-RVN® Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP).
- Product Information Sheet: EX-CELL® CD Insect Cell Medium
The EX-CELL® CD Insect Cell Medium is a specialized medium with a defined chemical composition, engineered to maximize the performance of insect cells. The formulation is animal-component free and has been formulated with L-Glutamine.
- White Paper: Development of a Novel Sf9 Rhabdovirus-Negative Cell Line (Sf-RVN®) and Companion Chemically Defined Medium
This whitepaper describes the development of a novel Sf9 Rhabdovirus-negative cell line, named Sf-RVN®, with companion chemically defined medium, the EXCELL® CD Insect Cell Medium. Combined, these two products form the Sf-RVN® Platform and offer a SfRhabdovirus free alternative for the BICS production of biologics.
- White Paper: Considerations for Bioreactor Process Development and Scale-up for Transient Transfection-based Lentivirus Production in Suspension
This white paper describes considerations for suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
- Tool: CHOZN® Omics Explorer
- White Paper: A Multifaceted Strategy for Viral Safety in AAV Processes
This white paper describes an approach to adventitious viral risk mitigation which encompasses this multifaceted approach defined as “prevent, detect, remove”.
- Brochure: VirusExpress® 293 Production Platform
Draw on our experience to get on the fast track through production.
- Data Sheet: VirusExpress® AAV Production Platform
The VirusExpress® Platform offers a transfectionbased solution to Adeno-Associated Viral (AAV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and a clinically relevant process dramatically reducing time to commercial production.
- Data Sheet: VirusExpress® Lentivirus Production Platform
The VirusExpress® Platform offers a transfection-based solution to lentiviral (LV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and process with proven performance at 50 L scale.
Sf-RVN® Platform For Improving the Safety Profile of Your Bioprocesses
The majority of Spodoptera frugiperda cells (such as Sf9) contain a rhabdovirus that is considered a process contaminant and must be eliminated during the bioprocess.
Our Sf-RVN® Platform offers a rhabdovirus-free alternative for bioprocesses and enhances risk mitigation.
The Sf-RVN® Platform is a synergy between a Sf9 rhabdovirus-negative cell line with companion chemically defined medium, specifically formulated to get excellent growth and productivity of the cell line.
Combined, the Sf-RVN® Insect Cell Line with companion EX-CELL® CD Insect Cell Medium provide a high performant platform optimal to produce recombinant proteins, viral vaccines such as viral like particles (VLP) and adeno-associated vectors (AAV) for gene therapy applications.
For more information on the Sf-RVN® Insect Cell Line or to order, please click here.
CHOZN® Platform for Production of Biologics and Recombinant Proteins
Our CHOZN® platform is a Chinese Hamster Ovary (CHO) mammalian cell expression system that allows for faster, simpler selection and scale-up of high-producing clones for production of biologics and therapeutic recombinant proteins. It includes:
- High-performing CHO cell lines
- Paired media and feeds
- Optimized expression vectors
- Robust protocols
Three CHOZN® cell lines are available:
- CHOZN® GS -/- cell line, enabling rapid cell line development without the need for amplification by MTX metabolic selection agent
- CHOZN® DHFR -/- cell line, enabling rapid cell line development by amplification via MTX metabolic selection agent
- CHOZN® CHO K1 cell line, enabling rapid cell line development with the metabolic selection agent of choice
Powered by CompoZr® Zinc Finger Nuclease (ZFN) technology, the well-known and widely adopted CHOZN® GS-/- cell line can help shorten cell line development time lines by up to eight weeks while ensuring excellent stability, with >75% of clones maintaining >70% titer of 60 generations.
Optimized expression vectors for the CHOZN® GS -/- cell line include:
- pCGS3.2 standard CHOZN® GS expression vector
- UCOE® GS expression vector using the chromatin opening UCOE® expression technology
Figure 1.The VirusExpress® Lentivirus Production Platform features a suspension adapted HEK293T cell line, chemically defined medium, and production process with proven performance.
VirusExpress® Lentiviral Production Platform
Our VirusExpress® platform offers a transfection-based solution for lentiviral (LV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and process with proven performance at clinically relevant scale. The platform can dramatically reduce time in process development and scale-up, either within your own facilities or using our contract manufacturing capabilities to speed your therapy to patients. Attributes include:
- cGMP banked and characterized cells
- Chemically defined medium
- Proven performance for cell growth, plasmid transfection, and lentiviral production in bioreactors at scale
- License available for clinical and commercial use
VirusExpress® 293T Lentiviral Production Cells are optimized for production of lentiviral vectors for gene therapy applications. Comprehensive user protocols guide you from seed train through at-scale transfection and virus production, allowing seamless scale-up. Robust quality documentation enables regulatory filings and commercialization of your gene-modified cell therapy.
Figure 2.The VirusExpress® AAV Production Platform features a suspension adapted HEK293 cell line, chemically defined medium, and production process with proven performance.
VirusExpress® AAV Production Platform
The VirusExpress® AAV Production Platform offers a transfection based solution to Adeno-Associated Viral (AAV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and a clinically relevant process dramatically reducing time to commercial production. VirusExpress® offers the flexibility to use either our contract manufacturing capabilities or your own facilities to speed your therapy to patients.
- A suspension adapted cell line of 293 AAV Production Cells optimized for production of AAV vectors for gene therapy applications.
- Chemically defined medium to alleviate animal origin and supply chain concerns.
- Cell banks manufactured according to GMP (21CFR210, 211, 600, 610) and fully characterized.
- Proven performance for cell growth, plasmid transfection, and AAV production in large-scale bioreactors at clinically and commercially relevant scale which yielded genome titers in excess of >2x1010 gc/mL.
- Licenses available for research, clinical and commercial use dependent on which phase of gene therapy development is needed.
- Reduced time in process development and scale-up by approximately 40%.
- Robust quality documentation to support regulatory filings and commercialization of your gene modified cell therapy or gene therapy.
We have the right cell lines, media products, manufacturing technologies and expertise to meet our customers’ timelines and their patients’ needs. By utilizing the VirusExpress® AAV Production Platform, we estimate you may be able to begin clinical manufacture in as little as 6 months as opposed to ~3 years for a custom cell line and process since clone selection, cell line development and GMP cell banking are complete, as well as substantial process development and largescale bioreactor development. Leveraging our expertise in viral vector manufacturing as well as utilizing our templated process solutions will help deliver your gene therapy to patients faster.
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