Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH.

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (2013-01-05)

C Almeida, N F Azevedo, J C Bento, N Cerca, H Ramos, M J Vieira, C W Keevil

PMID23288291

ABSTRACT

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

MATERIALS

Product Number

Brand

Product Description

MBD0040

Sigma-Aldrich

Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0039

Sigma-Aldrich

Proteus FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water