P3351

Protein L–Agarose from Peptostreptococcus magnus

recombinant, expressed in E. coli

• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• recombinant: expressed in E. coli
• matrix: 6% beaded agarose supplied as 50% slurry
• matrix activation: cyanogen bromide
• matrix attachment: amino
• capacity: 3-10 mg/mL binding capacity
• storage temp.: 2-8°C

Description

Application

Protein L-agarose is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, phosphorylation analysis, and protein A, G and L resins. Protein L-agarose has been used to provide evidence that antineuronal antibodies may contribute to neuronal dysfunction observed in a subset of patients with neurogenic chronic intestinal pseudoobstruction. Protein L agarose has also been used to evaluate a diabody to improve protection againse a potent scorpion neurotoxin.

Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding site. Recombinant Protein L contains four Ig-binding domains.

Preparation Note

Prepared with recombinant Peptostreptococcus magnus Protein L.

Safety Information

• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable