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A5955

Sigma-Aldrich

Antibiotic Antimycotic Solution (100×), Stabilized

suspension, suitable for cell culture, BioReagent

Synonym(s):

Penicillin-Streptomycin-Amphotericin B Suspension

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About This Item

MDL number:
MFCD00130520
UNSPSC Code:
12352207
NACRES:
NA.76

product name

Antibiotic Antimycotic Solution (100×), Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Quality Level

200

sterility

0.1 μm filtered

product line

BioReagent

form

suspension

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
fungi
yeast

application(s)

agriculture

Mode of action

cell wall synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

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General description

Antibiotic Antimycotic Solution is formulated to contain 10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and is solubilized in a proprietary citrate buffer. It is recommended for use in cell culture applications at 10mL/L. Antibioticantimycotic solution is generally used to prevent the growth of harmful microorganisms including bacteria and fungi in cell culture media.

Application

Antibiotic antimycotic solution has been used:
  • as a supplement in different types of cell culture media.
  • as a component of phosphate buffer saline (PBS) sterile solution to prevent contamination of periodontal ligament fibroblasts (PDLF) tissues that are isolated and cultured in the experiments.
  • as a component of storage solution for preserving tracheal segments harvested for subsequent experimental investigation.

Biochem/physiol Actions

Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out.

Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.

also commonly purchased with this product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of biocide addition on plantlet growth and contamination occurrence during the in vitro culture of blueberry
Sun YH, et al.
Journal of plant biotechnology, 42(2) (2015)
Kinue Aihara et al.
Kidney international, 64(4), 1283-1291 (2003-09-13)
Crystal formation and retention are critical events for the formation of kidney stones. Oxalate and calcium oxalate (CaOx) crystals are injurious to renal epithelium, and membranes of injured cells promote crystal adherence and retention. Calcium phosphate (CaP) is the most
Alessandro Taloni et al.
Scientific reports, 7(1), 3748-3748 (2017-06-18)
Classification of morphological features in biological samples is usually performed by a trained eye but the increasing amount of available digital images calls for semi-automatic classification techniques. Here we explore this possibility in the context of acrosome morphological analysis during
Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols
Bioengineering (Basel, Switzerland), 10(5) (2023)
Barbara Teuchner et al.
Cornea, 38(8), 1011-1016 (2019-04-02)
N-chlorotaurine (NCT) is an anti-infective belonging to the class of chloramines and an investigative drug for the topical treatment of keratoconjunctivitis. The aim of the present study was to demonstrate its efficacy against Acanthamoeba and Candida in corneas infected ex
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