Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

A3449

Sigma-Aldrich

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 19:1

Synonym(s):

30% Acrylamide/Bisacrylamide 19:1, Polyacrylamide solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770824
NACRES:
NA.25

product name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 19:1

sterility

0.2 μm filtered

Quality Level

200

product line

BioReagent

form

liquid

feed ratio

19:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Comparative performance of a novel herpes simplex virus type 2-specific enzyme-linked immunosorbent assay using a targeted chain oligopeptide, peptide 55
Al-Sulaiman AM, et al.
Clinical and Vaccine Immunology : CVI, 16(6), 931-934 (2009)
The
Ana Techniqs in Biotechnology (2011)
Kei Ihira et al.
Oncotarget, 8(8), 13509-13520 (2017-01-16)
EZH2 inhibition and reactivation of tumor suppressor microRNAs (miRNAs) represent attractive anti-cancer therapeutic strategies. We found that EZH2-suppressed let 7b and miR-361, two likely tumor suppressors, inhibited endometrial cancer (EC) cell proliferation and invasion, and abrogated cancer stem cell-like properties.
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)
Azis Boing Sitanggang et al.
Journal of food science and technology, 57(12), 4660-4670 (2020-10-23)
The influences of coagulation conditions on the characteristics of tofu have been investigated by many studies, with limited perspectives on the utilization of organic acid coagulants. Hence, this research aimed to study the psychochemical and functional properties of tofu coagulated
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service