MAB2252Z-I
Anti-Integrin β1 Antibody, clone N29 (Azide Free)
clone N29, Chemicon®, from mouse
Synonym(s):
CD29, MAB2252
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
N29, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgGκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
Specificity
Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
Immunogen
Jurkat T-leukemic cell line
Application
Immunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.
Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.
Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.
Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.
Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1.
Quality
Western Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.
Target description
88 kDa
Physical form
Format: Purified
Protein A purified
Purified cultured supernatant in PBS without preservatives.
Storage and Stability
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
Analysis Note
Control
Tonsil, human skin, human kidney tissue
U251 cell lysate
Tonsil, human skin, human kidney tissue
U251 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rania Hadjisavva et al.
Cell reports, 40(3), 111091-111091 (2022-07-21)
Cadherins and integrins are intrinsically linked through the actin cytoskeleton and are largely responsible for the mechanical integrity and organization of tissues. We show that cadherin clustering stimulates and spatially guides integrin activation. Adherens junction (AJ)-associated integrin activation depends on
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