A-004-M

Poly-ʟ-Ornithine

synthetic, liquid, 0.1 mg/mL, suitable for cell culture

• Synonym(s): Poly-L-Ornithine Solution (0.01%), A-004-M, Sigma-Aldrich
• UNSPSC Code: 12352209
• eCl@ss: 32160801
• NACRES: NA.75

Properties

• product name: Poly-L-Ornithine Solution (0.01%),
• needle size: 2.0 mm
• Quality Level: 100
• sterility: sterile
• form: liquid
• mol wt: M_w 78000 Da
• manufacturer/tradename: NovaSeptum^®
• concentration: 0.01 % (w/v); 0.1 mg/mL
• technique(s): cell attachment: suitable; cell culture | mammalian: suitable

Description

General description

Research Category: Stem Cell Research

Research Sub Category: Adhesion (CAMs)

Application

Poly-L-Ornithine Solution (0.01%) has been used to coat:

• glass-bottom plates for cell plating in a research study investigating the localization of cMET in MDA-MB-468 cells using confocal microscopy
• culture dishes for adult human neural stem cells (ahNSCs) primary culture
• 6-well plates for induced pluripotent stem cells (iPSCs) culture and neuronal differentiation

Quality

Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.

Physical form

Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.

Preparation Note

1. Thaw Poly-L-Ornithine solution at room temperature.

2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.

3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.

4. Allow the cell culture vessel to sit at room temperature overnight.

5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt. Avoid multiple freeze thaw cycles to maintain product integrity.

Legal Information

NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable