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Key Documents

07-471

Sigma-Aldrich

Anti-Daxx Antibody

serum, Upstate®

Synonym(s):

Anti-BING2, Anti-DAP6, Anti-EAP1, Anti-SMIM40

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse, hamster

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

hamster ... Daxx(101836409)
human ... DAXX(1616)
mouse ... Daxx(13163)
rat ... Daxx(140926)

Specificity

Daxx

Immunogen

GST fusion protein corresponding to residues 472-740 of human Daxx.

Application

Anti-Daxx Antibody is an antibody against Daxx for use in IP, WB & IC.

Quality

routinely evaluated by immunoblot in HeLa nuclear extract

Target description

110 kDa

Linkage

Replaces: 04-445

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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PML isoforms I and II participate in PML-dependent restriction of HSV-1 replication.
Cuchet, D; Sykes, A; Nicolas, A; Orr, A; Murray, J; Sirma, H; Heeren, J; Bartelt, A; Everett, RD
Journal of Cell Science null
Adenovirus type 5 early region 1B 55K oncoprotein-dependent degradation of cellular factor Daxx is required for efficient transformation of primary rodent cells.
Schreiner, S; Wimmer, P; Groitl, P; Chen, SY; Blanchette, P; Branton, PE; Dobner, T
Journal of virology null
Roger D Everett
Journal of virology, 90(1), 167-179 (2015-10-16)
Intrinsic immunity is an aspect of antiviral defense that operates through diverse mechanisms at the intracellular level through a wide range of constitutively expressed cellular proteins. In the case of herpesviruses, intrinsic resistance involves the repression of viral gene expression
Tetsuro Komatsu et al.
Journal of virology, 90(3), 1657-1667 (2015-11-27)
Promyelocytic leukemia protein nuclear bodies (PML-NBs) are subnuclear domains implicated in cellular antiviral responses. Despite the antiviral activity, several nuclear replicating DNA viruses use the domains as deposition sites for the incoming viral genomes and/or as sites for viral DNA
Steven McFarlane et al.
Virus research, 157(1), 47-53 (2011-02-12)
The control of human cytomegalovirus (HCMV) immediate early (IE) gene expression in infected human fibroblasts was compared with that in the U2OS human osteosarcoma cells. Viral IE expression was stimulated by the virion protein pp71 and repressed by the cell

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