SAB4200692

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

clone GST-2, purified from hybridoma cell culture

• Synonym(s): GST, Glutathione-S-Transferase
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GST-2, monoclonal
• form: buffered aqueous solution
• concentration: ~1 mg/mL
• technique(s): ELISA: suitable; dot blot: suitable; immunoblotting: 0.5 μg/mL; immunofluorescence: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.

Immunogen

purified recombinant GST fusion protein

Application

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• dot blotting
• autoradiography
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable