Unfortunately, this product is not tested for RNAse/DNAse activity. Please see the link below to review product B8667, which is an RNase/DNase-free BSA solution:
https://www.sigmaaldrich.com/product/sigma/b8667
A8412
Bovine Serum Albumin solution
7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture
Synonym(s):
Albumin solution from bovine serum, BSA solution
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About This Item
Biological source:
bovine
Assay:
7.0-8.0% protein basis (UV)
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤13.0 EU/mL Endotoxin level
Concentration:
7.5% in DPBS
Pricing and availability is not currently available.
biological source
bovine
Quality Segment
description
low endotoxin
sterility
sterile-filtered
product line
BioXtra
assay
7.0-8.0% protein basis (UV)
form
liquid
mol wt
66.4 kDa
purified by
cold ethanol and heatshock fractionation
origin
USA origin
concentration
7.5% in DPBS
technique(s)
cell culture | mammalian: suitable
impurities
≤13.0 EU/mL Endotoxin level
pH
6.5-7.5
foreign activity
Mycoplasma test (Negative), cell growth >50%
shipped in
ambient
storage temp.
2-8°C
General description
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Application
Qualified for use in cell culture applications.
Biochem/physiol Actions
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
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This Item | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| assay 7.0-8.0% protein basis (UV) | assay 28-32% protein basis (biuret) | assay 90-120 mg/mL protein basis (biuret) | assay ≥96% (agarose gel electrophoresis) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
| form liquid | form solution | form solution | form lyophilized powder |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is this product prepared in DPBS with or without Ca++ and Mg++?
1 answer -
Is this product RNAse/DNAse free? CAS No. 9048-46-8
1 answer-
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Is this product fatty acid free?
1 answer-
This product is not fatty acid free, it is fatty-acid depleted. The product is not tested for free fatty acid content. Please see several options below for fatty-acid free BSAs offered:
https://www.sigmaaldrich.com/product/sigma/a8806
https://www.sigmaaldrich.com/product/sigma/a7030
https://www.sigmaaldrich.com/product/sigma/a1595
https://www.sigmaaldrich.com/product/mm/126609Helpful?
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La référence A8412 a t-elle une date de retest ou d'expiration donnée par le fournisseur svp ?
1 answer-
This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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