10831

Histopaque^®-1083

sterile-filtered, density: 1.083

• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• sterility: sterile-filtered
• Quality Segment: 400
• form: liquid
• shelf life: Expiry date on the label.
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• density: 1.083
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Histopaque^®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque^®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Biochem/physiol Actions

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Features and Benefits

Histopaque^®-1083 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290,H315,H319
• pcodes: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
• Storage Class: 10 - Combustible liquids