Skip to Content
Merck
All Photos(1)

Documents

04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonym(s):

Staining kit for live/dead cells

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

suitability

suitable for fluorescence

Quality Level

100

storage temp.

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Kit Components Only

Product No.
Description
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Florian Rudnitzki et al.
Journal of biomedical optics, 17(5), 058003-058003 (2012-05-23)
When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal
Richardson N Leão et al.
PloS one, 5(11), e13833-e13833 (2010-11-17)
Pluripotent and multipotent stem cells hold great therapeutical promise for the replacement of degenerated tissue in neurological diseases. To fulfill that promise we have to understand the mechanisms underlying the differentiation of multipotent cells into specific types of neurons. Embryonic
Camille Raillon et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(10), 1085-1095 (2019-08-01)
The isolation, analysis, and enumeration of circulating tumor cells (CTCs) from cancer patient blood samples are a paradigm shift for cancer patient diagnosis, prognosis, and treatment monitoring. Most methods used to isolate and enumerate these target cells rely on the
Amin Hassanzadeh-Barforoushi et al.
Lab on a chip, 18(15), 2156-2166 (2018-06-21)
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized
S Yoshida et al.
Clinical nephrology, 49(5), 273-280 (1998-06-09)
Cardiovascular disease is one of the most common complications of dialysis and renal transplant patients, and high levels of AGE are present in end-stage renal failure. To address the potential involvement of AGE and growth factors in the pathophysiology of
View All Related Papers

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service