Ascentis^® Express C18 U/HPLC Columns

High Speed and High-Resolution HPLC & UHPLC Applications

Ascentis^® Express C18 is the first choice when starting a new separation method. The Fused-Core^® particle technology of Ascentis^® Express C18 enables high speed and high resolution separations on HPLC and UHPLC instruments. The column efficiency performs typically 40% higher in comparison to fully porous particulate columns with the same particle size.

The Ascentis^® Express C18 bonded phase is nonpolar in nature. It is best used with mobile phases that are mixtures of methanol and water or acetonitrile and water. Higher levels of the organic solvent component will typically reduce the retention of the sample compounds. Using elevated temperatures (e.g., 40–60 ºC) will reduce the viscosity of the mobile phase and allow the use of faster flow rates and lower column pressure for high sample throughput.

Ascentis® Express C18 Specifications
Silica:	Type B (High purity silica)
Particle Platform:	Superficially porous particles (SPP)
Phase Chemistry:	Dimethyloctadecyl
Particle Size:	2.0 µm / 2.7 µm / 5.0 µm
Pore Size:	90 Å
Carbon Load:	7.2% / 7.7% / 6.4%
Surface Area:	120 m²/g / 135 m²/g /90 m²/g
pH Range:	2-9
Max Temperature at Low pH:	60 °C
Max Temperature at High pH:	40 °C
Endcapped:	Yes

Brochure: Ascentis^® Express HPLC and UHPLC Columns

Featured Applications:

• Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3
• Accelerated Bioanalytical LC-MS-MS Using Ascentis^® Express Fused-Core HPLC Columns
• Separation of Aliphatic Amines

Reversed Phase C18 HPLC Columns with Fused-Core^® Technology

Ascentis^® Express C18 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core.  This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns

Materials

Ascentis^® Express C18

Length (mm) x I.D. (mm)	C18 (2 µm)	C18 (2.7 µm)	C18 (5 µm)
50 x 1.5		50629-U
100 x 1.5		50630-U
150 x 1.5		50636-U
20 x 2.1	50805-U	53799-U	50507-U
30 x 2.1	50809-U	53802-U	50508-U
50 x 2.1	50811-U	53822-U	50509-U
75 x 2.1	50812-U	53804-U	50511-U
100 x 2.1	50813-U	53823-U	50517-U
150 x 2.1	50814-U	53825-U	50518-U
250 x 2.1	on request	on request	50521-U
30 x 3.0	50815-U	53805-U	50522-U
50 x 3.0	50816-U	53811-U	50523-U
75 x 3.0	50817-U	53812-U	50525-U
100 x 3.0	50819-U	53814-U	50526-U
150 x 3.0	50821-U	53816-U	50527-U
250 x 3.0	on request	on request	50528-U
30 x 4.6	on request	53818-U	50529-U
50 x 4.6	on request	53826-U	50530-U
75 x 4.6	on request	53819-U	50533-U
100 x 4.6	on request	53827-U	50536-U
150 x 4.6	on request	53829-U	50537-U
250 x 4.6	on request	on request	50538-U
Guard columns
5 x 2.1	50822-U	53501-U	50539-U
5 x 3.0	on request	53504-U	50541-U
5 x 4.6		53508-U	50542-U

Figure 1. A guard column (53500-U) A) Finger tight guard cartridge holder, inlet, B) Finger tight guard cartridge holder, outlet, C) Auto-adjusting zero dead volume (ZDV), end fitting, D) Titanium hybrid ferrule