HPLC Analysis of Column Test Mixture on Ascentis® Express RP-Amide
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express RPA 15 cm x 4.6 mm, 5 μm particles (50773-U)
mobile phase
[A] 20 mM potassium phosphate, pH 7; [B] acetonitrile; (70:30, A:B)
flow rate
4.0 mL/min
pressure
4467 psi (308 bar)
column temp.
26 °C
detector
UV, 254 nm
injection
5 μL
sample
1. Uracil (0.016 mg/mL), 2. Benzamide (0.161 mg/mL), 3. Aniline (0.161 mg/mL), 4. Cinnamyl Alcohol (0.016 mg/mL), 5. Dimethyl Phthalate (0.323 mg/mL), (6. 2-Nitroaniline (0.081 mg/mL), 7. 4′-Bromoacetanilide (0.040 mg/mL), 8. 2,2′-Biphenol (0.161 mg/mL), 9. 4,4′-Biphenol (0.040 mg/mL), in 50/50 Water/Acetonitrile)
Description
Analysis Note
This application shows a test mixture of compounds of widely varying polarity on the 5μm RPA Ascentis Express column.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany