Characterization of recombinant β-amylases from Oryza sativa.

Characterization of recombinant β-amylases from Oryza sativa.

Bioscience, biotechnology, and biochemistry (2011-04-23)

Tomojiro Koide, Yasuo Ohnishi, Sueharu Horinouchi

ABSTRACT

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5-6.0, but they exhibited inferior heat stability to soybean β-amylase.

MATERIALS

Product Number

Brand

Product Description

A7130

Sigma-Aldrich

β-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)

A8781

Sigma-Aldrich

β-Amylase from sweet potato

A7005

Sigma-Aldrich

β-Amylase from sweet potato, Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)