ChIP for identification of p53 responsive DNA promoters.

ChIP for identification of p53 responsive DNA promoters.

Methods in molecular biology (Clifton, N.J.) (2012-11-15)

Jun-Ming Liao, Hua Lu

ABSTRACT

Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.

MATERIALS

Product Number

Brand

Product Description

R4642

Sigma-Aldrich

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

S3126

Sigma-Aldrich

Sperm nuclei from salmon, Type II-S