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Merck

CD4 T-cell epitope mapping.

Methods in molecular medicine (2001-01-01)
A A Delvig, J H Robinson
ABSTRACT

The majority of T cells recognize peptide epitopes bound to major histocompatibility complex (MHC)-encoded glycoproteins on the surface of professional antigen-presenting cells (APC), principally dendritic cells, macrophages, and B cells (1-3). Most T cells are specific for peptide epitopes in association with either classical MHC class Ia molecules (HLA-A, B, and C in humans and H2-K, D, and L in mice) in the case of CD8(+) T cells, or class II molecules (HLA-DR, DP, and DQ in humans and H2-A and E in mice) for CD4(+) T cells. However, a significant proportion of T cells recognize peptide antigens bound to nonclassical MHC class Ib molecules such as the human HLA-E (mouse analog Qa1) (4) and mouse H2-M3 (5). In addition, some T cells recognize not peptides but lipid or glycolipid antigens bound to nonclassical MHC class Ib molecules such as CD1 in both humans and mice (6).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder
Sigma-Aldrich
Concanavalin A from Canavalia ensiformis (Jack bean), Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture
Sigma-Aldrich
Fetal Bovine Serum, non-USA origin, sterile-filtered, suitable for cell culture
Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
TiterMax® Classic Adjuvant, liquid