Skip to Content
Merck
  • Blood plasma lipidome profile of dairy cows during the transition period.

Blood plasma lipidome profile of dairy cows during the transition period.

BMC veterinary research (2015-10-09)
S Imhasly, C Bieli, H Naegeli, L Nyström, M Ruetten, C Gerspach
ABSTRACT

The transition period of dairy cows, around parturition and the onset of lactation, involves endocrine and metabolic changes to compensate for an increased energy requirement aggravated by reduced feed intake. Transition cows adjust to the resulting negative energy balance with the mobilization of lipids from the adipose tissues yielding increased blood levels of non-esterified fatty acids and ketone bodies like β-hydroxybutyrate. To study the biochemical adaptations underlying this physiologic adjustment and possible pathologic derangements, we analyzed the blood plasma lipidome of transition cows by ultra-pressure liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry. The resulting data were processed by principal component analysis, revealing over 60 lipid masses that change in abundance over the test period ranging from two weeks before calving to four weeks postpartum. Further characterization of analytes by tandem mass spectrometry demonstrated that the concentration of triacylglycerides in plasma drops at the day of parturition whereas the plasma level of many phosphatidylcholines and two sphingomyelins increases steadily during early lactation. This newly identified shift in phospholipid composition delivers a potential biomarker to detect aberrant metabolic pathways in transition cows and also provides insights into how to prevent and treat associated disorders like fatty liver disease.

MATERIALS
Product Number
Brand
Product Description

Avanti
23:0 PC, 1,2-ditricosanoyl-sn-glycero-3-phosphocholine, powder
Avanti
23:0 PC, 1,2-ditricosanoyl-sn-glycero-3-phosphocholine, chloroform
Avanti
15:0 PE, 1,2-dipentadecanoyl-sn-glycero-3-phosphoethanolamine, powder