794-0715

HPLC Analysis of Paraquat and Diquat on SUPELCOSIL^™ LC-18 after SPE using ENVI^™-8 DSK

application for SPE, application for HPLC

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: drinking water adjusted to pH to 10.; sample preparation: ENVI-8 DSK, 47 mm (57172); SPE tube/cartridge: ENVI-8 DSK SPE Disk, 47 mm (57172); condition: 10 mL acetonitrile; 2 × 10 mL reagent water; 10 mL conditioning solvent A (5 g cetyl trimethyl ammonium bromide and 5 mL conc. ammonium hydroxide in 500 mL DI water, dilute to 1 L); (2 × 10 mL reagent water; 10 mL conditioning solvent B (10 g hexanesulfonic acid, sodium salt and 10 mL conc. ammonium hydroxide in 250 mL DI water, dilute to 500 mL)); sample addition: 250 mL water 100 mL/min; elution: 0.5-1.0 mL acetonitrile (to cover/solvate disk); 2 × 4 mL eluting solution (13.5 mL orthophosphoric acid and 10.3 mL diethylamine in 500 mL DI water, dilute to 1 L); column: SUPELCOSIL LC-18, 15 cm × 4.6 mm I.D., 5 μm particles (58230-U); mobile phase: 3.5 mL triethylamine and 1.0 g 1-hexane-sulfonic acid, sodium salt to 800 mL DI H₂O add orthophosphoric acid to pH 2.5, dilute to 1 L; flow rate: 1.0 mL/min; column temp.: 35 °C; detector: photodiode array, diquat - 308 nm, paraquat - 257 nm; injection: 100 μL
• suitability: application for HPLC; application for SPE
• application(s): environmental

Description

Application

Refer to US EPA Method 549.1 for full details

Sample Pre-treatment:
250 mL drinking water. Adjust water sample to pH to 10.5 ± 0.2 with 10% sodium hydroxide or 10% hydrochloric acid

Legal Information

ENVI is a trademark of Sigma-Aldrich Co. LLC

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC