11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• Synonym(s): chymotrypsin, protease
• Enzyme Commission number: 3.4.21.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine pancreas
• Quality Level: 100
• Assay: ≥90%
• form: lyophilized (salt-free)
• specific activity: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• mol wt: M_r 25 kDa
• packaging: pkg of 4 × 25 μg
• manufacturer/tradename: Roche
• technique(s): protein sequencing: suitable
• optimum pH: 7.0-9.0
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Specificity

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Application

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Quality

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Preparation Note

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitution

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable