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Key Documents

05-596

Sigma-Aldrich

Anti-Cdk2 Antibody, clone AN4.3

clone AN4.3, Upstate®, from mouse

Synonym(s):

Anti-CDKN2, Anti-p33(CDK2)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

AN4.3, monoclonal

species reactivity

mouse, Xenopus, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Cdk2(12566)

Specificity

cdk2, does not react with other cdks

Immunogen

Recombinant human cdk2

Application

0.5-2μg/ml of this lot detected cdk2 in HeLa nuclear extract. 4μg of a previous lot immunoprecipitated cdk2 from 500μg of mouse 3T3 nuclear extract.
Detect Cdk2 with Anti-Cdk2 Antibody, clone AN4.3 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Target description

33kDa

Physical form

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, and 30% glycerol.
Format: Purified
Protein G Chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K J Buchkovich et al.
Molecular biology of the cell, 7(9), 1443-1454 (1996-09-01)
Telomerase activity is involved in telomere length maintenance. Leukocytes, unlike many human somatic tissues, have detectable telomerase activity. These cells provide a normal human cell type in which to study telomerase. We studied the regulation of telomerase activity and the
APOLLON protein promotes early mitotic CYCLIN A degradation independent of the spindle assembly checkpoint.
Kikuchi, R; Ohata, H; Ohoka, N; Kawabata, A; Naito, M
The Journal of Biological Chemistry null
Sara Bolin et al.
Oncogene, 37(21), 2850-2862 (2018-03-08)
Medulloblastoma (MB) is the most common malignant brain tumor in children. MYC genes are frequently amplified and correlate with poor prognosis in MB. BET bromodomains recognize acetylated lysine residues and often promote and maintain MYC transcription. Certain cyclin-dependent kinases (CDKs)
Ji Eun Yu et al.
Molecular oncology, 16(11), 2214-2234 (2021-12-04)
Chitinase 3-like 1 (Chi3L1) is associated with various biological processes, such as inflammation, tissue repair, proliferation, cell survival, invasion, and extracellular matrix remodeling. Recent studies indicated that Chi3L1 is critical for cancer development and metastasis. In this study, we demonstrate
Michael P Walker et al.
The Journal of endocrinology, 207(2), 225-235 (2010-08-28)
Estrogens are potent mitogens for some target organs, such as the uterus, and cancers that develop in this organ might be linked to the proliferative action of these hormones. However, the mechanism by which estrogens influence the cell cycle machinery

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