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HPLC Analysis of Substituted Aromatic Compounds on Ascentis® Express Biphenyl

HPLC Analysis of Substituted Aromatic Compounds on Ascentis® Express Biphenyl application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

Uracil

≥99.0%

Acetophenone

analytical standard

Anisole

analytical standard

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 μm particles (64065-U)

mobile phase

[A] Water; [B]Methanol; (57:43 A:B)

flow rate

0.5 mL/min

pressure

7900 psi (545 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL

sample

Uracil (25 μg/mL), Benzonitrile (50 μg/mL), Acetophenone (25 μg/mL), Anisole (100 μg/mL),

Description

Analysis Note

This application demonstrate the separation of substituted aromatic compounds on Ascentis Express Biphenyl. These analytes highlight the unique selectivity of this stationary phase for this type of analytes using methanol reversed phase conditions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany