HPLC Analysis of Substituted Aromatic Compounds on Ascentis® Express Biphenyl
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CONDITIONS
column
Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 μm particles (64065-U)
mobile phase
[A] Water; [B]Methanol; (57:43 A:B)
flow rate
0.5 mL/min
pressure
7900 psi (545 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
1 μL
sample
Uracil (25 μg/mL), Benzonitrile (50 μg/mL), Acetophenone (25 μg/mL), Anisole (100 μg/mL),
Description
Analysis Note
This application demonstrate the separation of substituted aromatic compounds on Ascentis Express Biphenyl. These analytes highlight the unique selectivity of this stationary phase for this type of analytes using methanol reversed phase conditions.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany