Identification of the ron gene product as the receptor for the human macrophage stimulating protein.

Identification of the ron gene product as the receptor for the human macrophage stimulating protein.

Science (New York, N.Y.) (1994-10-07)

M H Wang, C Ronsin, M C Gesnel, L Coupey, A Skeel, E J Leonard, R Breathnach

ABSTRACT

Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.

MATERIALS

Product Number

Brand

Product Description

R7907

Sigma-Aldrich

RON (983-end), active, GST tagged human, PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution