Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Oncology letters (2015-01-27)

Chen Yu, Shen-Lin Liu, Ming-Hao Qi, Xi Zou, Jian Wu, Jing Zhang

PMID25621039

ABSTRACT

Guan Chang Fu Fang (GCFF) is a natural compound, which is extracted from three medicinal plants,

MATERIALS

Product Number

Brand

Product Description

W200603

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

W200611

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

71251

Supelco

Acetic acid, analytical standard

82762

Supelco

Methanol, analytical standard

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

F6627

Sigma-Aldrich

5-Fluorouracil, ≥99% (HPLC), powder

G5251

Sigma-Aldrich

DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H₂O

F8423

Sigma-Aldrich

Fluorouracil, meets USP testing specifications

45726

Sigma-Aldrich

Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)

606766

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

PHR1070

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

717975

Sigma-Aldrich

Methanol, NMR reference standard

1012772

USP

Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard

F0250000

Fluorouracil, European Pharmacopoeia (EP) Reference Standard

03738

Supelco

5-Fluorouracil, analytical standard

PHR1372

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

1279000

USP

Fluorouracil, United States Pharmacopeia (USP) Reference Standard

PHR1227

Supelco

Fluorouracil, Pharmaceutical Secondary Standard; Certified Reference Material

695092

Sigma-Aldrich

Acetic acid, glacial, ACS reagent, ≥99.7%

M1775

Sigma-Aldrich

Methanol, Absolute - Acetone free

154903

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

676780

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

338826

Sigma-Aldrich

Acetic acid, glacial, ≥99.99% trace metals basis

179337

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

179957

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

45754

Sigma-Aldrich

Acetic acid solution, suitable for HPLC

320390

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

494437

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

A6283

Sigma-Aldrich

Acetic acid, glacial, ReagentPlus^®, ≥99%