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  • Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits.

Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits.

Nanomedicine : nanotechnology, biology, and medicine (2015-02-06)
Kezheng Wang, Dipanjan Pan, Anne H Schmieder, Angana Senpan, Shelton D Caruthers, Grace Cui, John S Allen, Huiying Zhang, Baozhong Shen, Gregory M Lanza
ABSTRACT

A high r1 relaxivity manganese-gadolinium nanocolloid (αvβ3-MnOL-Gd NC) was developed and effectively detected atherosclerotic angiogenesis in rabbits fed cholesterol-rich diets for 12 months using a clinical MRI scanner (3T). 3D mapping of neovasculature signal intensity revealed the spatial coherence and intensity of plaque angiogenic expansion, which may, with other high risk MR bioindicators, help identify high-risk patients with moderate (40% to 60%) vascular stenosis. Microscopy confirmed the predominant media and plaque distribution of fluorescent αvβ3-MnOL-Gd NC, mirroring the MR data. An expected close spatial association of αvβ3-integrin neovasculature and macrophages was noted, particularly within plaque shoulder regions. Manganese oleate bioelimination occurred via the biliary system into feces. Gd-DOTA was eliminated through the bile-fecal and renal excretion routes. αvβ3-MnOL-Gd NC offers an effective vehicle for T1w neovascular imaging in atherosclerosis. From the clinical editor: Cerebrovascular accidents are a leading cause of mortality and morbidity worldwide. The acute formation of thrombus following atherosclerotic plaque rupture has been well recognized as the etiology of stroke. The authors studied microanatomical features of vulnerable atherosclerotic plaque in this article, in an attempt to identify those with high risk of rupture. Gadolinium-manganese hybrid nanocolloid (MnOL-Gd NC) was developed as a novel contrast agent for MRI. They show that this agent is effective in providing neovascular imaging.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Manganese, chips, thickness <2.0 mm, 99%
Sigma-Aldrich
Cholesterol, tested according to Ph. Eur.
Sigma-Aldrich
1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid, ≥97.0% (CHN)
Sigma-Aldrich
Cholesterol, from lanolin, ≥99.0% (GC)
Supelco
Cholesterol solution, certified reference material, 10 mg/mL in chloroform
Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Manganese, powder, ≥99.9% trace metals basis
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Manganese(II) chloride, beads, 98%
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Manganese(II) chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
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Manganese(II) chloride, powder and chunks, ≥99% trace metals basis
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Manganese, powder, −325 mesh, ≥99% trace metals basis
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Manganese(II) chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Cholesterol, from sheep wool, ≥92.5% (GC), powder
Sigma-Aldrich
Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%
Sigma-Aldrich
Cholesterol, Sigma Grade, ≥99%
Manganese, rod, 10mm, diameter 4.0mm, cast, 99.5%
Manganese, foil, not light tested, 25x25mm, thickness 0.02mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 50x50mm, thickness 0.009mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.002mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 50x50mm, thickness 0.0125mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 50x50mm, thickness 0.001mm, permanent polyester support, 98.7%
Manganese, foil, 50x50mm, thickness 2mm, hot-pressed, 99.95%
Manganese, foil, not light tested, 50x50mm, thickness 0.015mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 25x25mm, thickness 0.005mm, permanent polyester support, 98.7%
Manganese, rod, 50mm, diameter 4.0mm, cast, 99.5%
Manganese, foil, not light tested, 50x50mm, thickness 0.0025mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 50x50mm, thickness 0.01mm, permanent polyester support, 98.7%
Manganese, rod, 50mm, diameter 6.35mm, cast, 99.5%
Manganese, foil, not light tested, 50x50mm, thickness 0.003mm, permanent polyester support, 98.7%
Manganese, foil, not light tested, 50x50mm, thickness 0.025mm, permanent polyester support, 98.7%