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Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

Iron, IRMM^®, certified reference material, 0.5 mm wire

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

Iron, foil, light tested, 25x25mm, thickness 0.05mm, hard, 99.5%

Iron, foil, 6mm disks, thickness 0.5mm, hard, 99.5%

Iron, foil, 50mm disks, thickness 0.25mm, hard, 99.5%

Iron, foil, light tested, 100x100mm, thickness 0.025mm, as rolled, 99.99+%

Iron, foil, 8mm disks, thickness 0.05mm, as rolled, 99.99+%

Iron, foil, not light tested, 100x100mm, thickness 0.003mm, 99.85%

Iron, foil, 6mm disks, thickness 0.125mm, hard, 99.5%

Iron, foil, not light tested, 100x100mm, thickness 0.005mm, 99.85%

Iron, foil, 50mm disks, thickness 0.38mm, hard, 99.5%

Iron, foil, light tested, 25x25mm, thickness 0.075mm, hard, 99.5%

Iron, foil, 6mm disks, thickness 0.020mm, 99.85%

Iron, foil, light tested, 100x100mm, thickness 0.025mm, hard, 99.5%

Iron, foil, 6mm disks, thickness 0.125mm, as rolled, 99.99+%

Iron, foil, 8mm disks, thickness 0.05mm, hard, 99.5%

Iron, foil, 6mm disks, thickness 0.15mm, hard, 99.5%

Iron, foil, not light tested, 100x100mm, thickness 0.007mm, 99.85%

Iron, foil, 6mm disks, thickness 0.007mm, 99.85%