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Merck

Effects of phthalates on the human corneal endothelial cell line B4G12.

International journal of toxicology (2012-06-23)
Tanja Krüger, Yi Cao, Søren K Kjærgaard, Lisbeth E Knudsen, Eva C Bonefeld-Jørgensen
ABSTRACT

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

MATERIALS
Product Number
Brand
Product Description

Supelco
Benzyl butyl phthalate, analytical standard
Supelco
Benzyl butyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Benzyl butyl phthalate, 98%
Supelco
Di-n-octyl phthalate, analytical standard
Supelco
Diisodecyl phthalate, Selectophore, ≥99.0%
Supelco
Di-n-octyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Di-n-octyl phthalate, ≥98.0% (GC)