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Gel electrophoresis
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Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels
This page shows how to apply equilibrated Immobiline DryStrip gels
Formamide Molecular Biology Reagent
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
DNA Detection
Nancy-520 for DNA Detection and Quantitation
Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Paper-Bridge Loading
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
Applying Equilibrated Immobiline® DryStrip Gels
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Precast Agarose Gels for RNA Electrophoresis
Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.
Auto2D® Automated 2-D Gel Electrophoresis Device Protocol
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Sample Prep & Gel Electrophoresis Troubleshooting
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Troubleshooting 2-D DIGE Results
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.
Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
mPAGE®Lux Casting System for SDS-PAGE Gels
Refresh your gel casting workflow with ready-to-use SDS-PAGE gels in just 3 minutes. Discover faster, simpler, and safer gel casting methods.
Nucleic Acid Electrophoresis Protocols & Introduction
An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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