Skip to Content
Merck
All Photos(1)

Documents

T3449

Sigma-Aldrich

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Synonym(s):

Trypsin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.75

recombinant

expressed in corn

sterility

sterile-filtered

form

aqueous solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

shipped in

dry ice

storage temp.

−20°C

General description

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium

Features and Benefits

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Samia Rourou et al.
Biotechnology progress, 25(6), 1752-1761 (2009-09-22)
This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Katja Peschke et al.
EMBO molecular medicine, 14(4), e14876-e14876 (2022-02-05)
Despite the advance and success of precision oncology in gastrointestinal cancers, the frequency of molecular-informed therapy decisions in pancreatic ductal adenocarcinoma (PDAC) is currently neglectable. We present a longitudinal precision oncology platform based on functional model systems, including patient-derived organoids
Clay O Lacefield et al.
Cell reports, 26(8), 2000-2008 (2019-02-21)
The mammalian brain can form associations between behaviorally relevant stimuli in an animal's environment. While such learning is thought to primarily involve high-order association cortex, even primary sensory areas receive long-range connections carrying information that could contribute to high-level representations.

Articles

Cellular Fluorescence Imaging with Millicell® Cell Culture Inserts

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Protocols

Cancer Stem Cell Tumorsphere Formation Protocol

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service