CHO-K1.Cl6

15042901, hamster ovary, Polygonal

• UNSPSC Code: 41106514

Properties

• product name: CHO-K1.Cl6, 15042901, from hamster ovary
• biological source: hamster ovary
• description: CHO-K1, FcγRIIIa, Fc gamma receptor 3A, 158V, allotype, human IgG, IgG binding
• growth mode: Adherent
• morphology: Polygonal
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Stably transfected with a pcDNA3.1/Hygro(+) based vector with a human FcγRIIIa 158V cDNA insert that had been modified to enable expression as a GPI linked receptor

Cell Line Description

Clone of CHO-K1 cells expressing human FcγRIIIa of the 158V allotype anchored to glycosyl phosphatidylinositol

Culture Medium

MEM (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 10% FBS (F2442) with 600ug/ml hygromycin B

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO₂/95%air; 37°C

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.CI6 (ECACC 15042901)

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.