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SHM02

Sigma-Aldrich

MISSION® ExpressMag® 96-Well Magnetic Kit

Increases transduction efficiency

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

product line

MISSION®

shelf life

≥2 yr at ~4 °C

storage temp.

2-8°C

General description

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

MISSION® ExpressMag® 96-Well Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product is specifically designed to work with 96-well plates. The kit has been documented to enhance MISSION lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag system.
MISSION® ExpressMag® 96-Well Magnetic Kit has been used in hepatitis C virus (HCV) replicon luciferase assay.[1]

Warning

Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

Legal Information

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • SHM05MISSION® ExpressMag® 96-Well Magnet, Increases transduction efficiencySDS
  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiencySDS

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Certificates of Analysis (COA)

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E J Murray et al.
Antimicrobial agents and chemotherapy, 55(9), 4311-4319 (2011-06-29)
The current standard of care for hepatitis C virus (HCV) patients is cotreatment with human alpha interferon (IFN-α) and ribavirin. The host factor USP18 functions to regulate the interferon signaling pathway by acting as an off-switch. In order to understand
Knockdown of USP18 increases alpha 2a interferon signaling and induction of interferon-stimulating genes but does not increase antiviral activity in Huh7 cells
Murray EJ, et al.
Antimicrobial Agents and Chemotherapy, 55(9), 4311-4319 (2011)

Questions

1–6 of 6 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What cell lines have been transduced successfully using ExpressMag Beads?

    1 answer

    1. The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.

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  3. What other viruses have been successfully introduced into cells via the use of ExpressMag beads?

    1 answer

    1. Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.

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  4. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  5. What is the typical fold increase in transduction efficiency when using ExpressMag beads?

    1 answer

    1. The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.

      Helpful?

  6. What MISSION products have been successfully introduced into cells using ExpressMag® beads?

    1 answer

    1. MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.

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