MABT911F

Anti-Tspan-33 Antibody, clone 29A6.2 Antibody, FITC conjugate

clone 29A6.2, from mouse, FITC conjugate

• Synonym(s): Tetraspanin-33, Penumbra, mPen, Proerythroblast new membrane
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 29A6.2, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• technique(s): flow cytometry: suitable
• isotype: IgMκ
• NCBI accession no.: NP_666285.1
• UniProt accession no.: Q8R3S2
• target post-translational modification: unmodified
• Gene Information: mouse ... Tspan33(232670)

Description

General description

Tetraspanin-33 (UniProt: Q8R3S2; also known as Tspan-33, Penumbra, mPen, Proerythroblast new membrane) is encoded by the Tspan33 (also known as Pen) gene (Gene ID: 232670) in murine species. Tetraspanins are evolutionarily conserved proteins and thus far 33 of them have been described in human and murine species. Tspan-33 is a multi-pass, disulfide-linked homodimeric membrane protein of the tetraspanin (TM4SF) family. It has been reported that Tspan-33 is highly expressed in activated B cells. Its expression has also been reported in malignant B cells. Two isoforms of Tspan-33 have been described that are produced by alternative splicing. Tspan-33 plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. Tspan-33 contains four transmembrane domains (aa 25-45, 65-85, 97-117, 236-256), three cytoplasmic domains (aa 1-24, 86-96, 257-283), and two extracellular domains (aa 46-64, 118-235). Several tetraspanins interact with specific membrane proteins via their second extracellular domain (aa 118-235) and incorporate them into tetraspanin-enriched microdomains to modulate the effectiveness of signal transduction by those membrane proteins. (Ref.: Luu, VP., et al. (2013). Clin. Immunol. 149(3): 388-399).

Specificity

Clone 29A6.2 specifically detects Tspan-33 and targets an epitope with in the extracellular domain.

Immunogen

BaF3 cells expressing full-length murine Tspan-33 fused with a Myc-His tag at the C-terminus.

Epitope: extracellular domain

Application

Anti-Tspan-33, clone 29A6.2, FITC conjugate, Cat. No. MABT911F, is a mouse monoclonal antibody that detects Tetraspanin-33 and has been tested for use in Flow Cytometry.

Research Category
Cell Structure

Quality

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.5 µg of this antibody detected Tspan-33 in 500,000 cells per mL mouse splenocytes.

Target description

31.55 kDa calculated.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgM in PBS with 0.1% sodium azide, with 1.5% BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.