Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. The serine-threonine kinase ROCK-1 is a down-stream effector of Rho, and is involved in signal transduction pathways that regulate cell morphology, motility, and tumor progression through the reorganization of the actin cytoskeleton.
Specificity
This antibody recognizes ROCK-1 and ROCK-2.
Immunogen
Synthetic peptide corresponding to human ROCK-1, 90.5% homology with ROCK-2
Application
This Anti-ROCK-1/2 Antibody is validated for use in WB for the detection of ROCK-1/2.
Quality
Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 1:500 dilution of this antibody detected ROCK-1/2 on 10 µg of A431 cell lysate.
Target description
158 kDa
Linkage
Replaces: 04-1121
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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