S5761

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

• Synonym(s): Sodium hydrogen carbonate, soda bicarbonate
• Linear Formula: NaHCO₃
• CAS Number: 144-55-8
• Molecular Weight: 84.01
• Beilstein: 4153970
• EC Number: 205-633-8
• MDL number: MFCD00003528
• UNSPSC Code: 12161702
• PubChem Substance ID: 24899673
• NACRES: NA.75
• Assay: 99.5-100.5%
• grade: for molecular biology
• form: powder
• solubility: water: soluble 100 mg/mL

Properties

• grade: for molecular biology
• Quality Level: 300
• product line: BioReagent
• Assay: 99.5-100.5%
• form: powder
• quality: 300
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• pH: 6.8-7.4
• pKa (25 °C): (1) 6.37, (2) 10.25 (carbonic acid)
• solubility: water: soluble 100 mg/mL
• density: 2.16 g/mL at 25 °C (lit.)
• SMILES string: [Na+].OC([O-])=O
• InChI: 1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
• InChI key: UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

General description

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Application

Sodium bicarbonate has been used:

• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
• as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
• as a component of medium used for cell culture of human embryonic kidney cells[4]
• as a component of Beltsville thawing solution used for the dilution of semen[5]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves