HPLC Analysis of Norepinephrine and 3,4-Dihydroxyphenylglycol on Discovery® HS F5
CONDITIONS
column
Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567561-U)
mobile phase
50 mM ammonium formate, pH to 3.0 with formic acid
flow rate
1.0 mL/min
column temp.
35 °C
detector
UV, 266 nm
injection
10 μL
sample
50 μg/mL each (NE and DHPG) in 5% methanol in mobile phase
Beschreibung
Rechtliche Hinweise
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany