HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix® WCX NP1.7
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
Proteomix® WCX-NP1.7 Weak Cation Exchange Column
L × I.D. 3 cm × 4.6 mm, 1.7 μm particle size, stainless steelCONDITIONS
column
Proteomix WCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 μm particles (Z777179)
mobile phase
(A) 20 mM sodium acetate, pH 5.15; (B) A + 1 M LiCl
gradient
12% to 40% B in 6.7 min; to 100% B in 0.1 min; held for 2 min
flow rate
(1.5 mL/min)
pressure
3335-3916 psi (230 - 270 bar)
detector
UV 280 nm
injection
25 μL
sample
1 mg/mL papain digested MAb 321
Beschreibung
Rechtliche Hinweise
Proteomix is a registered trademark of Sepax Technologies