HPLC Analysis of Bilirubin and Biliverdin on Discovery^® C18

column

Discovery C18, 10 cm × 2.1 mm I.D., 5 μm particles (569220-U)

mobile phase

[A] 10 mM ammonium acetate (pH unadjusted) [B] 90:10, methanol:(100 mM ammonium acetate)

flow rate

0.2 mL/min

column temp.

ambient

detector

UV, 405 nm

injection

1 μL

sample

as indicated (in 50% MeOH, 1% NH₄OH)