Direkt zum Inhalt

Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.

Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.

Nature plants (2015-09-01)

Erik Jonsson, Moé Yamada, Ronald D Vale, Gohta Goshima

ZUSAMMENFASSUNG

The molecular motors kinesin and dynein drive bidirectional motility along microtubules (MTs) in most eukaryotic cells. Land plants, however, are a notable exception, because they contain a large number of kinesins but lack cytoplasmic dynein, the foremost processive retrograde transporter. It remains unclear how plants achieve retrograde cargo transport without dynein. Here, we have analysed the motility of the six members of minus-end-directed kinesin-14 motors in the moss

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Sigma-Aldrich

Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%

Sigma-Aldrich

MOPS, ≥99.5% (titration)

Sigma-Aldrich

Ethylenglykol-bis(2-aminoethylether)-N,N,N′,N′-Tetraessigsäure, for molecular biology, ≥97.0%

Sigma-Aldrich

MOPS, BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

Sigma-Aldrich

Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%

Sigma-Aldrich

Chloroform, ReagentPlus^®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Paclitaxel, from semisynthetic, ≥98%

Sigma-Aldrich

MOPS, BioXtra, ≥99.5% (titration)

Sigma-Aldrich

Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Paclitaxel, from Taxus brevifolia, ≥95% (HPLC), powder

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Ethylenglykol-bis(2-aminoethylether)-N,N,N′,N′-Tetraessigsäure, ≥97.0%

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

Sigma-Aldrich

Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)

SAFC

MOPS

Sigma-Aldrich

Ethylenglykol-bis(2-aminoethylether)-N,N,N′,N′-Tetraessigsäure, BioXtra, ≥97 .0%

Sigma-Aldrich

Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

Sigma-Aldrich

Paclitaxel, from Taxus yannanensis, powder

Sigma-Aldrich

MOPS, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%