Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films.

Polyelectrolyte multilayer (PEM) film can modify the surface properties of materials to improve cellular responses. In this study poly(diallyldimethylammonium chloride) (PDADMAC), poly(sodium 4-styrene sulfonate) (PSS) and poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (PSS-co-MA) were assembled into PEM {(PDADMAC/PSS)(4)/PDADMAC+PSS-co-MA} film on glass surfaces and its ability on affecting osteoblast functions were examined. PSS-co-MA film showed an increase roughness and more wettable surface as compared to glass. When the osteoblast cell line, MC3T3-E1, was seeded on the surfaces, no differences were observed in cell attachment or spreading on either PSS-co-MA film or glass at 4-16 hours. However, increases in alkaline phosphatase activity (day-5 and 7) and the expression of osteocalcin mRNA/protein at day-13 were observed. Cells cultured on PSS-co-MA film developed a faster rate of calcium deposition at day-15 compared to control. In conclusion, PSS-co-MA film enhanced osteoblast differentiation and could be used to promote mineralization and improve osseointegration for dental implants.