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Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.

Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.

European journal of mass spectrometry (Chichester, England) (2013-07-12)

Gabi Drochioiu, Laura Ion, Catalina Ciobanu, Laura Habasescu, Ionel Mangalagiu

ZUSAMMENFASSUNG

The interaction between copper ions and gamma-L-glutamyl-L-cysteinyl-glycine [glutathione (GSH)] molecules may lead to the formation of the physiologically occurring Cu[I)-[GSH]2 and Cu(II)-GSSG complexes. Since glutathione depletion in neurons and aberrant copper metabolism have been implicated in several neurodegenerative disorders, we studied here the interaction of GSH with copper ions (Cu2+) by electrospray ionization ion trap mass spectrometry (ESI-IT-MS). Besides, we extended this approach to pH in excess of 10 by adding ethanolamine to the solution being investigated. As a result, the ESI-IT-MS spectra revealed novel aspects regarding the speciation of copper-glutathione complex.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

L-Glutathion, oxidiert, ≥98% (HPLC)

Sigma-Aldrich

Ethanolamin, ≥99%

Sigma-Aldrich

Kupfer, powder, 99.999% trace metals basis

Sigma-Aldrich

Kupfer, foil, thickness 0.25 mm, 99.98% trace metals basis

Sigma-Aldrich

Ethanolamin, purified by redistillation, ≥99.5%

Sigma-Aldrich

Kupfer, powder, <425 μm, 99.5% trace metals basis

Sigma-Aldrich

Ethanolamin, ≥98%

Sigma-Aldrich

Kupfer, wire, diam. 1.0 mm, ≥99.9%

Sigma-Aldrich

L-Glutathion, oxidiert, lyophilized powder

Sigma-Aldrich

Kupfer, powder, <75 μm, 99%

Sigma-Aldrich

Ethanolamin, ACS reagent, ≥99.0%

Sigma-Aldrich

Kupfer, nanopowder, 40-60 nm particle size (SAXS), ≥99.5% trace metals basis

Sigma-Aldrich

Kupfer, powder (spheroidal), 10-25 μm, 98%

Sigma-Aldrich

L-Glutathion, oxidiert, BioXtra, ≥98%

Sigma-Aldrich

Kupfer, wire, diam. 0.25 mm, 99.999% trace metals basis

Sigma-Aldrich

Kupfer, nanopowder, 60-80 nm particle size (SAXS), ≥99.5% trace metals basis

Sigma-Aldrich

Kupfer, foil, thickness 0.025 mm, 99.98% trace metals basis

Sigma-Aldrich

Kupfer, powder (dendritic), <45 μm, 99.7% trace metals basis

Sigma-Aldrich

Kupfer, foil, ≥99.8% (complexometric)

Sigma-Aldrich

Kupfer, turnings, purum p.a., ≥99.0%

Sigma-Aldrich

Kupfer, wire, diam. 0.64 mm, 99.995% trace metals basis

Sigma-Aldrich

Kupfer, foil, thickness 0.5 mm, 99.98% trace metals basis

Sigma-Aldrich

Kupfer, shot, −3-+14 mesh, 99%

Sigma-Aldrich

Ethanolamin, liquid, BioReagent, suitable for cell culture, ≥98%

Sigma-Aldrich

Kupfer, beads, 2-8 mm, 99.9995% trace metals basis

Sigma-Aldrich

Kupfer, foil, thickness 1.0 mm, 99.999% trace metals basis

Supelco

Ethanolamin, analytical standard

Sigma-Aldrich

Kupfer, wire, diam. 2.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Kupfer, beads, 2-8 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Kupfer, ACS reagent, granular, 10-40 mesh, ≥99.90%