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  • Isolation and characterization of a novel 2-sec-butylphenol-degrading bacterium Pseudomonas sp. strain MS-1.

Isolation and characterization of a novel 2-sec-butylphenol-degrading bacterium Pseudomonas sp. strain MS-1.

Biodegradation (2009-08-26)
Tadashi Toyama, Noritaka Maeda, Manabu Murashita, Yong-Cheol Chang, Shintaro Kikuchi
ZUSAMMENFASSUNG

A novel bacterium capable of utilizing 2-sec-butylphenol as the sole carbon and energy source, Pseudomonas sp. strain MS-1, was isolated from freshwater sediment. Within 30 h, strain MS-1 completely degraded 1.5 mM 2-sec-butylphenol in basal salt medium, with concomitant cell growth. A pathway for the metabolism of 2-sec-butylphenol by strain MS-1 was proposed on the basis of the identification of 3 internal metabolites-3-sec-butylcatechol, 2-hydroxy-6-oxo-7-methylnona-2,4-dienoic acid, and 2-methylbutyric acid-by gas chromatography-mass spectrometry analysis. Strain MS-1 degraded 2-sec-butylphenol through 3-sec-butylcatechol along a meta-cleavage pathway. Degradation experiments with various alkylphenols showed that the degradability of alkylphenols by strain MS-1 depended strongly on the position (ortho > meta = para) of the alkyl substitute, and that strain MS-1 could degrade 2-alkylphenols with various sized and branched alkyl chain (o-cresol, 2-ethylphenol, 2-n-propylphenol, 2-isopropylphenol, 2-sec-butylphenol, and 2-tert-butylphenol), as well as a dialkylphenol (namely, 6-tert-butyl-m-cresol).

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
2,4-Di-tert.-butylphenol, 99%
Sigma-Aldrich
2-tert-Butylphenol, 99%
Sigma-Aldrich
3-tert-Butylphenol, 99%
Sigma-Aldrich
2-sec-Butylphenol, 98%
Sigma-Aldrich
4-Butylphenol, AldrichCPR