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Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.

Methods in molecular biology (Clifton, N.J.) (2020-10-20)
Jacob F Warner, Eric Röttinger
ZUSAMMENFASSUNG

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
Phenol:Chloroform:Isoamylalkohol 25:24:1, gesättigt mit 100 mM TRIS pH 8,0; 1 mM EDTA, Supplied with Equilibration buffer, for molecular biology